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Wounds.:Gelam蜂蜜促进角膜上皮细胞增殖期的潜力!

2017-07-06 cuiguizhong MedSci原创

用0.0015%GH处理的培养基增加了离体角膜上皮细胞增殖,同时保留了其表型特征。本研究证实了GH型局部治疗对超声角膜损伤的潜在发展。

近日,来自马来西亚科邦沙医科大学生理系的Hui CK团队在Wounds.杂志上发表了他们的最近研究:Gelam蜂蜜促进角膜上皮细胞增殖期的潜力。

在本项研究中,作者们调查了Gelam蜂蜜(GH)促进离体角膜上皮细胞(CECs)增殖及其对表型特征的影响的潜在益处。

他们从兔角膜(n = 6)中分离角膜上皮细胞。通过MTT(3- [4,5-二甲基噻唑基-2] -2,5-二苯基四唑溴化物)测定法测定基础培养基(BM)和角膜培养基(CM)中离体角膜上皮细胞增殖的最佳剂量。通过相差显微镜、实时聚合酶链反应、免疫细胞化学和流式细胞技术分别评估了离体角膜上皮细胞的形态、基因和蛋白表达、细胞周期分析。

研究结果表明,在0.0015%GH补充培养基(BM + 0.0015%GH; CM + 0.0015%GH)中培养的角膜上皮细胞具有正常多边形形态的最佳增殖能力。 Gelam蜂蜜根据细胞质CK3蛋白表达增强细胞角蛋白3(CK3)基因表达,同时保持离体角膜上皮细胞的正常细胞周期。

由此,作者们得出结论:用0.0015%GH处理的培养基增加了离体角膜上皮细胞增殖,同时保留了其表型特征。本研究证实了GH型局部治疗对超声角膜损伤的潜在发展。

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    2018-03-07 lxg951
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    2017-07-08 snowpeakxu
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  6. [GetPortalCommentsPageByObjectIdResponse(id=2042350, encodeId=640a20423501e, content=<a href='/topic/show?id=37a090929f5' target=_blank style='color:#2F92EE;'>#角膜上皮#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90929, encryptionId=37a090929f5, topicName=角膜上皮)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d8e0333, createdName=zhangyxzsh, createdTime=Wed Jan 17 20:14:00 CST 2018, time=2018-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2068732, encodeId=26ee2068e32f1, content=<a href='/topic/show?id=3be4200324e' target=_blank style='color:#2F92EE;'>#上皮细胞增殖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20032, encryptionId=3be4200324e, topicName=上皮细胞增殖)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6a6043, createdName=guoyibin, createdTime=Sat Nov 25 06:14:00 CST 2017, time=2017-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2057602, encodeId=891e205e6020d, content=<a href='/topic/show?id=c02b106e29a' target=_blank style='color:#2F92EE;'>#LAM#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10672, encryptionId=c02b106e29a, topicName=LAM)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04ce158, createdName=lxg951, createdTime=Wed Mar 07 22:14:00 CST 2018, time=2018-03-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1349076, encodeId=105913490e6e1, content=<a href='/topic/show?id=4337909284a' target=_blank style='color:#2F92EE;'>#角膜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90928, encryptionId=4337909284a, topicName=角膜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=568152, createdName=snowpeakxu, createdTime=Sat Jul 08 12:14:00 CST 2017, time=2017-07-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1533826, encodeId=021015338262c, content=<a href='/topic/show?id=4be9200308c' target=_blank style='color:#2F92EE;'>#上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20030, encryptionId=4be9200308c, topicName=上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=30bd35, createdName=一叶知秋, createdTime=Sat Jul 08 12:14:00 CST 2017, time=2017-07-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1540894, encodeId=a93c15408946f, content=<a href='/topic/show?id=ba2ee78630b' target=_blank style='color:#2F92EE;'>#细胞增殖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77863, encryptionId=ba2ee78630b, topicName=细胞增殖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f58613291856, createdName=晓辰, createdTime=Sat Jul 08 12:14:00 CST 2017, time=2017-07-08, status=1, ipAttribution=)]
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