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Medicine:维吾尔族人群种植体周龈下微生物多样性分析

2018-02-03 MedSci MedSci原创

本研究旨在探讨维吾尔族种植体周围龈下菌群的多样性和群落结构。从40名健康对照组(健康种植体)和病例组(种植体周围炎)维吾尔族人群收集龈沟液。提取DNA,在Illumina Miseq测序平台上进行16SrRNA V4-V5区域的测序。构建并分析16SrRNA基因克隆文库。结果显示,40个样本中共获得733759个有效标签。通过Uparse软件与Silva-16S数据库进行对比,最后收获了263个操

本研究旨在探讨维吾尔族种植体周围龈下菌群的多样性和群落结构。

从40名健康对照组(健康种植体)和病例组(种植体周围炎)维吾尔族人群收集龈沟液。提取DNA,在Illumina Miseq测序平台上进行16SrRNA V4-V5区域的测序。构建并分析16SrRNA基因克隆文库。

结果显示,40个样本中共获得733759个有效标签。通过Uparse软件与Silva-16S数据库进行对比,最后收获了263个操作分类单元(对照组135个,病例组128个)。两组之间在门、类、次序、家族和属水平上存在差别的细菌分别是放线菌、放线菌门、巴斯德氏菌、莫拉菌科和不动杆菌。对照组和病例组间分布存在明显差异的优势菌属包括弧菌、弯曲杆菌、颗粒菌、不动杆菌、微球菌和莫拉菌属。基于chao多样性指数的α多样性分析表明,两组之间微生物多样性存在明显差异。主坐标分析分析表明这两组之间的细菌群落结构存在明显差异。聚类分析显示病例组球菌丰度较高,而对照组普氏菌属丰度较高。

综上所述,该研究结果表明,维族健康种植体与种植体周围炎的龈下菌群多样性差异有统计学意义。莫拉克斯氏菌属、球菌和不动杆菌属可能是维吾尔族人群中引起种群种植体周围炎的优势菌。

原始出处:

Gao X, Zhou J, Sun X, Li X, Zhou Y. Diversity analysis of subgingival microbial bacteria in peri-implantitis in Uygur population. Medicine (Baltimore). 2018 Feb;97(5):e9774. doi: 10.1097/MD.0000000000009774.

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    2018-03-06 kalseyzl
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    2018-04-24 jeanqiuqiu
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encryptionId=698b69296c2, topicName=生物多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c77e138, createdName=kalseyzl, createdTime=Fri Mar 02 00:35:00 CST 2018, time=2018-03-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701902, encodeId=5b191e01902ac, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Sat Nov 10 15:35:00 CST 2018, time=2018-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343753, encodeId=55371343e53a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1470923, encodeId=7e5f14e092301, content=<a href='/topic/show?id=507043296fe' target=_blank style='color:#2F92EE;'>#多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=43296, encryptionId=507043296fe, topicName=多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b6a7095267, createdName=neurosurgeon, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577588, encodeId=e7c115e7588f4, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1608523, encodeId=8c701608523ce, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=)]
  5. [GetPortalCommentsPageByObjectIdResponse(id=1773690, encodeId=85f81e7369000, content=<a href='/topic/show?id=5f014e1852' target=_blank style='color:#2F92EE;'>#CIN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4718, encryptionId=5f014e1852, topicName=CIN)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a9a038523631, createdName=kalseyzl, createdTime=Tue Mar 06 17:35:00 CST 2018, time=2018-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858848, encodeId=a2be185884805, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, 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encryptionId=698b69296c2, topicName=生物多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c77e138, createdName=kalseyzl, createdTime=Fri Mar 02 00:35:00 CST 2018, time=2018-03-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701902, encodeId=5b191e01902ac, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Sat Nov 10 15:35:00 CST 2018, time=2018-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343753, encodeId=55371343e53a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1470923, encodeId=7e5f14e092301, content=<a href='/topic/show?id=507043296fe' target=_blank style='color:#2F92EE;'>#多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=43296, encryptionId=507043296fe, topicName=多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b6a7095267, createdName=neurosurgeon, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577588, encodeId=e7c115e7588f4, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1608523, encodeId=8c701608523ce, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=)]
    2018-11-10 huangdf
  6. [GetPortalCommentsPageByObjectIdResponse(id=1773690, encodeId=85f81e7369000, content=<a href='/topic/show?id=5f014e1852' target=_blank style='color:#2F92EE;'>#CIN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4718, encryptionId=5f014e1852, topicName=CIN)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a9a038523631, createdName=kalseyzl, createdTime=Tue Mar 06 17:35:00 CST 2018, time=2018-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858848, encodeId=a2be185884805, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, 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  7. 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  8. 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createdName=jeanqiuqiu, createdTime=Tue Apr 24 15:35:00 CST 2018, time=2018-04-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1824882, encodeId=521a18248825d, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Sun Jan 13 21:35:00 CST 2019, time=2019-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1798507, encodeId=c0eb1e9850717, content=<a href='/topic/show?id=698b69296c2' target=_blank style='color:#2F92EE;'>#生物多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69296, encryptionId=698b69296c2, topicName=生物多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c77e138, createdName=kalseyzl, createdTime=Fri Mar 02 00:35:00 CST 2018, time=2018-03-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701902, encodeId=5b191e01902ac, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Sat Nov 10 15:35:00 CST 2018, time=2018-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343753, encodeId=55371343e53a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1470923, encodeId=7e5f14e092301, content=<a href='/topic/show?id=507043296fe' target=_blank style='color:#2F92EE;'>#多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=43296, encryptionId=507043296fe, topicName=多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b6a7095267, createdName=neurosurgeon, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577588, encodeId=e7c115e7588f4, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1608523, encodeId=8c701608523ce, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=)]
  9. [GetPortalCommentsPageByObjectIdResponse(id=1773690, encodeId=85f81e7369000, content=<a href='/topic/show?id=5f014e1852' target=_blank style='color:#2F92EE;'>#CIN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4718, encryptionId=5f014e1852, topicName=CIN)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a9a038523631, createdName=kalseyzl, createdTime=Tue Mar 06 17:35:00 CST 2018, time=2018-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858848, encodeId=a2be185884805, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Tue Apr 24 15:35:00 CST 2018, time=2018-04-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1824882, encodeId=521a18248825d, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Sun Jan 13 21:35:00 CST 2019, time=2019-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1798507, encodeId=c0eb1e9850717, content=<a href='/topic/show?id=698b69296c2' target=_blank style='color:#2F92EE;'>#生物多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69296, encryptionId=698b69296c2, topicName=生物多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c77e138, createdName=kalseyzl, createdTime=Fri Mar 02 00:35:00 CST 2018, time=2018-03-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701902, encodeId=5b191e01902ac, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Sat Nov 10 15:35:00 CST 2018, time=2018-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343753, encodeId=55371343e53a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1470923, encodeId=7e5f14e092301, content=<a href='/topic/show?id=507043296fe' target=_blank style='color:#2F92EE;'>#多样性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=43296, encryptionId=507043296fe, topicName=多样性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b6a7095267, createdName=neurosurgeon, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577588, encodeId=e7c115e7588f4, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1608523, encodeId=8c701608523ce, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Feb 05 01:35:00 CST 2018, time=2018-02-05, status=1, ipAttribution=)]
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