J Endod：活髓治疗生物材料的血管生成活性评估

2018-05-13 lishiting MedSci原创

这篇研究的目的是为了评估生物活性材料的血管生成特性，包括：white mineral trioxide aggregate (WMTA), calcium hydroxide (Ca[OH]2), Geristore (Den-Mat, Santa Maria, CA)和nano WMTA。

这篇研究的目的是为了评估生物活性材料的血管生成特性，包括：white mineral trioxide aggregate (WMTA)， calcium hydroxide (Ca[OH]2)， Geristore (Den-Mat， Santa Maria， CA)和nano WMTA。

研究将WMTA， Ca(OH)2， Geristore和nano WMTA片分散至2 mL Milli-Q (Millipore， ThermoFisher， Hanover Park， IL)去离子水中，离心以获得2 mL的上清液。将35孔的聚乙二醇水凝胶列阵分为5组，每组7孔(n = 7)。将小鼠磨牙内皮细胞(ECs) 置于水凝胶列阵内。每个样本的洗脱预备：生长培养基内按照1：3的比例稀释，并加入水凝胶列阵。EC培养基用作对照。对于脉络膜新生血管 (CNV) 模型的构建，将35只6周龄雌鼠进行辐照并分为5组，每个样本的洗脱液(2 μL)或生理盐水（对照）在激光治疗当天和1周后进行玻璃体内注射。通过ImageJ software (National Institutes of Health， Bethesda， MD)计算结节的平均数量、水凝胶列阵内分支的整个长度和CNV的平均面积，并通过1-way方差分析和post hoc Tukey honest检验进行统计学评估。

结节数量结果的比对显示，对照组> Geristore > nano WMTA > WMTA > Ca(OH)2。对于整个分支长度和CNV面积的比对结果显示，Geristore > 对照组 > nano WMTA > WMTA > Ca(OH)2。

结论：所有测试材料均展现出极小的抗血管生成活性，而Geristore和nano WMTA与WMTA 和Ca(OH)2相比则展现出较高的促血管生成活性。

原始出处：

Saghiri MA， Asatourian A， et al. Hydrogel Arrays and Choroidal Neovascularization Models for Evaluation of Angiogenic Activity of Vital Pulp Therapy Biomaterials. J Endod. 2018 May；44(5)：773-779. doi： 10.1016/j.joen.2018.02.008. Epub 2018 Mar 27.

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2018-06-25 aids213

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2018-08-13 1249857fm32暂无昵称

#生物材#

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2018-05-14 dongjia2013

#生物材料#

66 0
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2018-05-14 121832a9m88暂无昵称

学习了

75 0
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2018-05-13 1209e435m98(暂无昵称)

学习了.谢谢分享

78 0
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2018-05-13 龙胆草

学习谢谢分享

82 0
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2018-05-13 明月清辉

谢谢分享.学习了

84 0
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2018-05-13 wzb521zf

一起学习学习

81 0
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2018-05-13 飛歌

学习了很有用

42 0
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2018-05-13 执着追梦

学习.谢谢分享

44 0