J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

2020-02-09 lishiting MedSci原创

这篇研究的目的是为了评估poly lactic-co-glycolic acid (PLGA)支架负载编码纤维生长因子-2 DNA的质粒(pFGF-2)对体外培养的人牙周膜细胞(hPDLCs)的作用，并评估PLGA/pFGF-2支架促进比格犬牙撕脱模型中牙周膜(PDL)再生的能力。

这篇研究的目的是为了评估poly lactic-co-glycolic acid (PLGA)支架负载编码纤维生长因子-2 DNA的质粒(pFGF-2)对体外培养的人牙周膜细胞(hPDLCs)的作用，并评估PLGA/pFGF-2支架促进比格犬牙撕脱模型中牙周膜(PDL)再生的能力。研究通过静电纺丝法制备PLGA和PLGA/pFGF-2支架。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察分析支架材料的特点。通过dsDNA HS检测PLGA/pFGF-2支架的质粒释放情况。通过CCK-8法检测hPDLCs在2种支架上的活性和增殖情况，并通过RT-qPCR检测I型胶原和scleraxis的表达变化。将撕脱牙的牙根分别覆盖2种支架材料并再植入比格犬的牙槽窝内。采用HE和Masson染色评估PLGA/pFGF-2支架材料促进PDL再生的效果。结果显示，2种支架材料均可见平滑且均匀的纤维，并且PLGA/pFGF-2支架中可见质粒随机均匀的分布。dsDNA HS分析结果显示，PLGA/pFGF-2支架在超过21天的时间持续稳定的释放pFGF-2 123 ng，未见明显的爆发式释放。与PL

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2020-05-20 ms8435843352634314

#FGF-2#

59 0
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2020-02-10 wodejia-dayu

#acid#

63 0
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2021-01-05 xqptu

#牙周膜#

59 0
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2020-12-27 xlxchina

#CID#

65 0
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2020-09-27 feather89

#PE#

50 0
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2020-02-11 zhouqu_8

#牙齿#

44 0
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2020-02-11 医者仁心

#牙周#

47 0
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2020-02-11 12498f6em12暂无昵称

#编码#

52 0

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J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

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J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

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Nat Commun：突破！科学家成功开发出新版本干细胞或具有强大的治疗潜力