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Photochem Photobiol:氯己定与抗菌光动力疗法联合使用对钛片表面牙龈卟啉单胞菌的灭菌作用

2018-12-10 MedSci MedSci原创

既往研究已经提出各种抗菌方法来治疗种植体周围炎,但结果有限。本研究旨在评估联合应用氯己定二葡萄糖酸盐(CHX)和先前被牙龈卟啉单胞菌生物膜污染的钛表面的抗菌光动力疗法(aPDT)的灭菌效力。牙龈卟啉单胞菌生物膜在32个抛光和32个喷砂的大颗粒酸蚀刻(SLA)钛表面上生长。将钛片分成如下四组:(i)浸入磷酸盐缓冲盐水(PBS)中,(ii)浸入0.2%CHX中,(iii)施用aPDT,(iv)浸入0.

既往研究已经提出各种抗菌方法来治疗种植体周围炎,但结果有限。本研究旨在评估联合应用氯己定二葡萄糖酸盐(CHX)和先前被牙龈卟啉单胞菌生物膜污染的钛表面的抗菌光动力疗法(aPDT)的灭菌效力。

牙龈卟啉单胞菌生物膜在32个抛光和32个喷砂的大颗粒酸蚀刻(SLA)钛表面上生长。将钛片分成如下四组:(i)浸入磷酸盐缓冲盐水(PBS)中,(ii)浸入0.2%CHX中,(iii)施用aPDT,(iv)浸入0.2%CHX中,随后aPDT 。通过微生物培养分析和扫描电子显微镜(SEM)和共聚焦激光扫描显微镜(CLSM)成像确定残留细菌。组合方案(CHX + aPDT)在抛光和SLA表面上根除牙龈卟啉单胞菌最有效(P <0.05)。四组抛光钛盘与SLA组之间残留的牙龈卟啉单胞菌数量差异无统计学意义(P> 0.05)。

总之,在本研究的限制下,CHX的先前应用和随后的aPDT的组合技术是可减少抛光和SLA钛表面中牙龈卟啉单胞菌数量的有效方法。

原始出处:

Cai Z, Li Y, et al., Disinfect Porphyromonas gingivalis biofilm on titanium surface with combined application of chlorhexidine and antimicrobial photodynamic therapy. Photochem Photobiol. 2018 Nov 27. doi: 10.1111/php.13060.

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  5. 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style='color:#2F92EE;'>#光动力#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=28733, encryptionId=0da528e33ce, topicName=光动力)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2b6213920685, createdName=luckyshitiancai_42705688, createdTime=Tue Dec 11 15:55:00 CST 2018, time=2018-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354991, encodeId=2ebe3549911d, content=感谢小编为我们准备了如此丰盛的精神大餐,同时也向作者致谢!认真学习了,点赞!, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Mon Dec 10 08:57:44 CST 2018, time=2018-12-10, status=1, 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    2019-04-17 liuxiaona
  6. 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  7. 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style='color:#2F92EE;'>#光动力#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=28733, encryptionId=0da528e33ce, topicName=光动力)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2b6213920685, createdName=luckyshitiancai_42705688, createdTime=Tue Dec 11 15:55:00 CST 2018, time=2018-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354991, encodeId=2ebe3549911d, content=感谢小编为我们准备了如此丰盛的精神大餐,同时也向作者致谢!认真学习了,点赞!, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Mon Dec 10 08:57:44 CST 2018, time=2018-12-10, status=1, ipAttribution=)]
    2018-12-10 ylzr123

    感谢小编为我们准备了如此丰盛的精神大餐,同时也向作者致谢!认真学习了,点赞!

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