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MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo

Zhang, X; Lv, S; Luo, ZH; Hu, YF; Peng, X; Lv, J; Zhao, SS; Feng, JQ; Huang, GJ; Wan, QL; Liu, J; Huang, HX; Luan, B; Wang, D; Zhao, XY; Lin, Y; Zhou, QH; Zhang, ZN; Rong, ZL

Rong, ZL (corresponding author), Southern Med Univ, Sch Basic Med Sci, Canc Res Inst, Guangzhou 510515, Peoples R China.; Zhou, QH (corresponding author), Jinan Univ, Zhuhai Peoples Hosp, Zhuhai Inst Translat Med, Zhuhai 519000, Peoples R China.; Zhou, QH; Rong, ZL (corresponding author), Jinan Univ, Fac Med Sci, Biomed Translat Res Inst, Guangzhou 510632, Peoples R China.; Zhang, ZN (corresponding author), Tongji Univ, Shanghai East Hosp, Translat Med Ctr Stem Cell Therapy, Sch Life Sci & Techn

NUCLEIC ACIDS RESEARCH, 2021; 49 (7): 4171

Abstract

CRISPR-mediated gene activation (CRISPRa) is a promising therapeutic gene editing strategy without inducing DNA double-strand breaks (DSBs). However, ......

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