EBM：以崭新之生物医学造影技术研究癌症发展模式

由Gillies博士所领导的亚历桑纳癌症中心及摩非特癌症中心(Moffitt Cancer Center)的研究团队目前已经证实了，藉由崭新的核磁共振造影技术，以非侵入性的方式监控乳癌模式动物中癌细胞的生长。此一量化造影技术测量与癌细胞生长息息相关的癌组织内水份扩散。此一结果已发表于2012年11月号《实验生物医学》期刊，将有助于发展崭新的癌症研究模式。

Gillies博士指出，先前在缺乏影像技术的情况下，大多数癌症模式的发展者并未能准确监控癌症的发展进程。我们的实验结果指出了，癌症组织中的水份能提供许多关于癌症发展的讯息。更准确的说，水份的流动一般会被生物性的阻碍如细胞膜所阻隔，因为水份的流动性降低将可作为细胞密度与癌症模式生长速率的指标。

发展崭新的癌症模式对于现今的癌症研究非常的重要。目前为止，藉由取自病人检体之癌细胞作为癌症研究的模式仍为是十分耗费人力却鲜少有进展的研究方式。本研究之共同主持人，Pagel博士指出，非侵入性量化造影技术十分具有潜力可以加速以及促进建立癌症发展的模式，以提供前瞻性的癌症研究之用。

《实验生物医学》期刊主编古德曼博士指出，Cillies博士及其研究团队的实验结果证实了，权重扩散式核磁造影技术为乳癌模式提供了一个量化癌症生长的方式。此类非侵入性量测方法，将十分有助于发展乳癌的新癌症模式来增进发展暨改进新的治疗方法。

与癌症相关的拓展阅读：

• Onco & Cancer Res：袁增强等Hippo信号通路和癌症及细胞衰老研究获进展
• Am J Epidemiol：用体质指数判定癌症风险或需重新思考
• Nat Chem Biol：探究O-葡萄糖转移酶在癌症糖尿病等疾病中的作用
• CEBP：癌症幸存者生活质量或受影响
• Am J Epidemiol：夜班或增加男性患癌症风险 更多信息请点击：有关癌症更多资讯

Monitoring the development of xenograft triple-negative breast cancer models using diffusion-weighted magnetic resonance imaging
ABSTRACT
Evaluations of tumor growth rates and molecular biomarkers are traditionally used to assess new mouse models of human breast cancers. This study investigated the utility of diffusion weighted (DW)-magnetic resonance imaging (MRI) for evaluating cellular proliferation of new tumor models of triple-negative breast cancer, which may augment traditional analysis methods. Eleven human breast cancer cell lines were used to develop xenograft tumors in severe combined immunodeficient mice, with two of these cell lines exhibiting sufficient growth to be serially passaged. DW-MRI was performed to measure the distributions of the apparent diffusion coefficient (ADC) in these two tumor xenograft models, which showed a correlation with tumor growth rates and doubling times during each passage. The distributions of the ADC values were also correlated with expression of Ki67, a biomarker of cell proliferation, and hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor receptor-2 (VEGFR2), which are essential proteins involved in regulating aerobic glycolysis and angiogenesis that support tumor cell proliferation. Although phosphatase and tensin homolog (PTEN) levels were different between the two xenograft models, AKT levels did not differ nor did they correlate with tumor growth. This last result demonstrates the complexity of signaling protein pathways and the difficulty in interpreting the effects of protein expression on tumor cell proliferation. In contrast, DW-MRI may be a more direct assessment of tumor growth and cancer cell proliferation.

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